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Cloning and Expression
Enzymes
DraI
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DraI
DraI from New England Biolabs
Be the first to write a review!
Citations:
(23)
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Supplier Page from
New England Biolabs for
DraI
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Company Info
Citations
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Product Specs
Item
DraI
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
R0129L
Concentration
20 u/µL
Quantity
10000 units
Applications
DNA Manipulation
Brand/Trademark
New England Biolabs Inc (NEB)
Type
Restriction Enzyme
Temperature
-20 C
Sequence
TTT/AAA
Add to Compare List
New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (23)
(1)
Grk7 but not Grk1 undergoes cAMP-dependent phosphorylation in zebrafish cone photoreceptors and mediates cone photoresponse recovery to elevated cAMP
The Journal of biological chemistry
December 1, 2022
Chrispell JD, Xiong Y, Weiss ER.
Sanger sequencing
(Genewiz), plasmid
clones
containing the correct insert were linearized with
DraI
(#
R0129S
,
New England Biolabs
) and sgRNA was synthesized
in vitro
using a
MAXIscript T7 Transcription Kit
(#
AM1312
,
(2)
A transcriptional roadblock protects yeast centromeres
Nucleic Acids Research
August 12, 2022
Sabrine Hedouin, Glennis A Logsdon, Jason G Underwood, Sue Biggins
7.6, 0.1% NP-40, 175 mM K-glutamate and 15% glycerol) supplemented with 0.1% BSA, 10 U of
DraI
(
NEB
, #
R0129S
) and 2.5 U of
SspI
(
NEB
, #
R0132S
) and incubated at 30°C for 15 min to allow the cleavage of
kinetochore
-unbound
(3)
Structural basis of RNA conformational switching in the transcriptional regulator 7SK RNP
Molecular Cell
May 1, 2022
Yuan Yang, Shiheng Liu, Sylvain Egloff, Catherine D. Eichhorn, Tanya Hadjian, James Zhen, Tamás Kiss, Z. Hong Zhou, Juli Feigon
2005",N/A
Spermidine
,
Sigma-Aldrich
,Cat. #
S2626
BamHI-HF
restriction enzyme,
NEB
,Cat #
R3136M
DraI
restriction enzyme,
NEB
,Cat #
R0129S
T4 Polynucleotide Kinase
,
NEB
,Cat #
M0201L
Dulbecco’s Modified Eagle Medium (DMEM),"GIBCO, Invitrogen",Cat
(4)
The Australian dingo is an early offshoot of modern breed dogs
Science Advances
April 22, 2022
Matt A. Field et al.
Supermix (Bio-Rad), 1 μl of each 20× primer/probe, 1 μl of Dra I restriction enzyme (
New England Biolabs
#
R0129S
), 5 μl of DNA template (4 ng/μl), and 2 μl of ddH2O. Copy number data were rounded to the nearest whole
(5)
Chromosome-length genome assembly and structural variations of the primal Basenji dog (Canis lupus familiaris) genome
BMC Genomics
December 1, 2021
Richard J. Edwards et al.
Supermix (Bio-rad), 1 μl of each 20x primer/probe, 1 μl of
DraI
restriction enzyme (
New England BioLabs
#
R0129S
), 5 μl of DNA template (4 ng/μl) and 2 μl ddH2O. Primer sequence for Amy2B: forward 5′-CCAAACCTGGACGGACATCT-3′
(6)
Microscopic examination of spatial transcriptome using Seq-Scope
Cell
June 24, 2021
Cho CS, Xi J, Si Y, Park SR, Hsu JE, Kim M, Jun G, Kang HM, Lee JH.
nuclease-free water 3 times. Then, the flow cell was treated with
DraI
enzyme cocktail (1U
DraI
enzyme (#
R0129
,
NEB
) in 1X
CutSmart buffer
) in 37 °C overnight to completely cut out the P5 sequence and expose oligo-dT.
(7)
DNA length tunes the fluidity of DNA-based condensates
Biophysical journal
April 6, 2021
Muzzopappa F, Hertzog M, Erdel F.
(NEB,
R3136
), and
KpnI
(NEB, R0142); or 4)
AciI
(
NEB
,
R0551
),
HindIII
(
NEB
,
R0104
), and
DraI
(
NEB
,
R0129
). The 1 kb and 5 kb fragments were generated by
PCR
using the oligonucleotides LambdaFW and Lambda1_rv/Lambda5_rv
(8)
Topological stress is responsible for the detrimental outcomes of head-on replication-transcription conflicts
Cell Reports
March 2, 2021
Kevin S. Lang, Houra Merrikh
and recombinant proteins",,
EcoRV-HF
,
NEB
,R0195
HindIII-HF
,
NEB
,
R0104
EcoRI-HF
,
NEB
,R0101
DraI
,
NEB
,
R0129
RNase H
,
NEB
,
M0297
Protein A Sepharose,GE,GE17-0780-01 Critical commercial
assays
,, Nextera XT
DNA Library Preparation
(9)
Cell Cycle-Dependent EBNA1-DNA Cross-Linking Promotes Replication Termination at oriP and Viral Episome Maintenance
Cell
February 1, 2021
Jayaraju Dheekollu, Andreas Wiedmer, Kasirajan Ayyanathan, Julianna S. Deakyne, Troy E. Messick, Paul M. Lieberman
M0264S
Mung bean nuclease
,
NEB
,Cat#
M0250S
T4PNK,
NEB
,Cat#
M0201S
DpnI
,
NEB
,
R0176L
BamHI
,
NEB
,
R3136L
Dra1,
NEB
,
R0129L
DNase I
,
Roche
,Cat# 04-716728-001 RNaseA,
Invitrogen
,Cat#
12091-021
Benzonase,
Millipore
,Cat#
71206-3
KEY
(10)
A cell-free antibody engineering platform rapidly generates SARS-CoV-2 neutralizing antibodies
bioRxiv : the preprint server for biology
October 30, 2020
Chen X, Gentili M, Hacohen N, Regev A.
agarose gel extraction. The purified
ligation
product was then digested by
DraI
(
New England Biolabs
,
R0129S
) and a fragment of ~680 bp in size was purified by DNA agarose gel extraction to get the final VHH library,
(11)
The testis-specifically expressed gene
Trim69
is not essential for fertility in mice
Journal of biomedical research
August 21, 2020
He X, Xie W, Li H, Cui Y, Wang Y, Guo X, Sha J.
plasmid which has been Bsa I (
NEB
, USA,
R0535S
)-digested. The plasmid was digested with Dra I (
NEB
,
R0129S
) and purified using MinElute PCR Purification Kit (
Qiagen
,
28004
). SgRNAs were produced using the MEGAshortscript
(12)
Trait-associated noncoding variant regions affect TBX3 regulation and cardiac conduction
eLife
July 16, 2020
Jan Hendrik van Weerd, Rajiv A Mohan, Karel van Duijvenboden, Ingeborg B Hooijkaas, Vincent Wakker, Bastiaan J Boukens, Phil Barnett, Vincent M Christoffels
(Invitrogen). sgRNA (pDR274) and Cas9 (MLM3613) expression vectors were linearized with
DraI
(
NEB
,
R0129S
) and
PmeI
(
NEB
,
R0560S
), respectively. sgRNA and Cas9
in vitro transcription
was performed using the
(13)
Caspase-8-Dependent Inflammatory Responses Are Controlled by Its Adaptor, FADD, and Necroptosis
Immunity
June 16, 2020
Tummers B, Mari L, Guy CS, Heckmann BL, Rodriguez DA, Rühl S, Moretti J, Crawford JC, Fitzgerald P, Kanneganti TD,
et al
.
001 Puromycin,Sigma-Aldrich,Cat# P-8833 Q-VD(OMe)-Oph,APExBIO,Cat# A8165 Restriction enzyme
DraI
,
New England BioLabs
,Cat#
R0129
Restriction enzyme
SacI
,
New England BioLabs
,Cat# R0156 Sterile PBS,
Gibco
,Cat# 14190-144
Streptavidin-HRP
,ThermoShandon,Cat#
(14)
Trio deep-sequencing does not reveal unexpected off-target and on-target mutations in Cas9-edited rhesus monkeys
Nature Communications
December 1, 2019
Xin Luo et al.
transcribed by the MEGAshortscript Kit (
Ambion
,
AM1354
) after the vectors were linearized by
DraI
(
NEB
,
R0129S
). SgRNAs were purified by the
MEGAclear Kit
(
Ambion
,
AM1908
). Cas9 mRNAs were transcribed by the T7
(15)
Screening Mycobacterium tuberculosis Secreted Proteins Identifies Mpt64 as a Eukaryotic Membrane-Binding Bacterial Effector
mSphere
June 5, 2019
Stamm CE, Pasko BL, Chaisavaneeyakorn S, Franco LH, Nair VR, Weigele BA, Alto NM, Shiloh MU.
2021 by 2a01:4f9:3a:1a85::2. Stamm et al. linearized with AflII and
DraI
(catalog no. R0520 and
R0129
;
New England Biolabs
[NEB]) and transformed into
E. coli
EL350/phAE87 by
electroporation
. Phagemid DNA
(16)
Usherin defects lead to early-onset retinal dysfunction in zebrafish
Experimental Eye Research
August 1, 2018
Margo Dona et al.
oligo and surrounding sequences were excised from the pDR274 vector using
DraI
(New England Bioloabs, #
R0129S
). The excised DNA band (284 basepairs) was subsequently used as a template for
in vitro transcription
(17)
Efficient Gene Transfer and Gene Editing in Sterlet (Acipenser ruthenus)
Frontiers in Genetics
April 6, 2018
Ji Chen, Wei Wang, Zhaohui Tian, Ying Dong, Tian Dong, Hua Zhu, Zuoyan Zhu, Hongxia Hu, Wei Hu
confirmed by
PCR
using M13(-47) primer and oligo R. The reconstructed plasmid was digested by
DraI
(
NEB
,
R0129
), and a 285 bp DNA fragment was recycled and cleaned up. This fragment was then used as a template in an
(18)
CRISPR/Cas9‐Directed Gene Editing for the Generation of Loss‐of‐Function Mutants in High‐Throughput Zebrafish F
0
Screens
Current Protocols in Molecular Biology
July 6, 2017
Sunita S. Shankaran, Timothy J. Dahlem, Brent W. Bisgrove, H. Joseph Yost, Martin Tristani‐Firouzi
Cat# 6022 DH5alpha chemically competent
E. coli
cells
DraI
with
CutSmart buffer
from
NEB
. Cat#
R0129S
HiScribe T7 Quick High Yield
RNA Synthesis
Kit form
NEB
. Cat#
E2050S
RNase free dH2O
Qiagen
RNeasy
(19)
Efficient generation of multiple-gene knockout pig parthenogenetic embryos by using CRISPR/Cas9 system and rapid estimation of knockout efficiency by single blastocyst genotyping
SCIENTIA SINICA Vitae
March 1, 2016
JiMin GAO, NanNan KANG, HongQuan CHEN
sgRNA 靶位点各设计一对引物进行
PCR
扩增, 引物序列见 表 3. 1.2 体外转录 体外转录参考 zhou 等人 [22] 的方法, 简述如下: sgRNA 的体外转录: ( ⅰ) 用 DraⅠ(
NEB
,
R0129S
) 线性化构建好的 sgRNA 载体后, 用
PCR
的方式将 305 https://engine.scichina.com/doi/10.1360/N052016-00034 康难难等:
(20)
A cell free system for functional centromere and kinetochore assembly Authors
Nature Protocols
October 1, 2012
Annika Guse, Colin J. Fuller, Aaron F. Straight
28106
) Restriction enzymes
EcoRI
,
DraI
,
XbaI
,
HaeII
,
AvaI
(
New England BioLabs
, catalogue # R0101,
R0129
, R0145,
R0107
, R0152)
NEB
2-log DNA ladder (New England BioLabs, catalogue # N3200) Agarose (GeneMate
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DraI from New England Biolabs
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